Abstract
The imiquimod-poly(2-(2-methoxyethoxy)ethyl methacrylate) hydrogel (poly(MEO2MA) hydrogel) dressing was developed for the keloid therapy application. Four groups of the hydrogels, including the imiquimod-poly(MEO2MA) hydrogel, crosslinked with 0.2 mol %, 0.4 mol %, 0.6 mol %, and 0.8 mol % of di(ethylene glycol) dimethacrylate cross-linker (DEGDMA), were synthesised and characterised for fabricating the imiquimod-poly(MEO2MA) hydrogel pad. The lower critical solution temperature (LCST) of the poly(MEO2MA) hydrogel was measured at approximately 28 °C and was used as a trigger to control the imiquimod loading and release. The loaded amounts of the imiquimod in the poly(MEO2MA) hydrogel, crosslinked with 0.2 mol % and 0.8 mol % of DEGDMA, were about 27.4 μg and 14.1 μg per 1 mm3 of the hydrogel, respectively. The imiquimod-release profiles of two samples were characterised in a phosphate buffered saline (PBS) solution at 37 °C and the released imiquimod amount were about 45% and 46% of the total loaded imiquimod. The Cell Counting Kit-8 (CCK-8) assay was utilised to analyse the cell viability of keloid fibroblasts cultured on the samples of imiquimod-poly(MEO2MA) hydrogel, crosslinked with 0.2 mol % and 0.8 mol % of DEGDMA. There was around a 34% decrease of the cell viabilities after 2 days, compared with the pure-poly(MEO2MA) hydrogel samples. Therefore, the developed imiquimod-poly(MEO2MA) hydrogel dressing can affect the proliferation of keloid fibroblasts. It should be possible to utilise the hydrogel dressing for the keloid therapy application.
Highlights
Wounds are typically referred to as the damage of tissue or outer skin injuries caused by physical means
The imiquimod releasing profiles of the fabricated imiquimod-poly(MEO2 MA) dressings were cultured on the fabricated dressing, and Cell Counting Kit-8 (CCK-8) assay was applied to study were measured in phosphate buffered saline (PBS) solution at 37 ◦ C
The lower critical solution temperature (LCST) and swelling/deswelling properties of the synthesised poly(MEO2MA) hydrogel with different molar feed ratios of cross-linker were characterised for fabricating the imiquimodpoly(MEO2MA) hydrogel dressing
Summary
Wounds are typically referred to as the damage of tissue or outer skin injuries caused by physical means. The imiquimod is an immune response modifier and stimulates interferon, a proinflammatory cytokine that can effectively make collagen collapse It has been suggested as an effective way to reduce the recurrence of keloid [9]. Whichhydrophilic is an insoluble hydrophilic polymer, emerged as a promising material for which is an insoluble polymer, has emerged as ahas promising material for wound dressing. It provides high-water content (70–90%), which maintains a moist condition for the tissue-grown the tissue-grown microenvironment. The imiquimod releasing profiles of the fabricated imiquimod-poly(MEO2 MA) dressings were cultured on the fabricated dressing, and Cell Counting Kit-8 (CCK-8) assay was applied to study were measured in phosphate buffered saline (PBS) solution at 37 ◦ C.
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