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https://doi.org/10.1006/fsim.1995.0033
Copy DOIJournal: Fish and Shellfish Immunology | Publication Date: Jul 1, 1995 |
Citations: 24 |
Two monoclonal antibodies (MAbs) against seabass ( Dicentrarchus labrax) immunoglobulin (IgM) were obtained after lymphocytic hybridisation made from mouse splenocytes. Screening, using an indirect enzyme-linked immunosorbent assay (ELISA), revealed two probes which had a specific activity against seabass IgM and did not react with turbot, sea bream, eel and rainbow-trout IgM. These probes belonged to a sub-class of IgG2a and recognised exclusively the heavy chains (H) of seabass IgM on immunoblotting. Their immunological activity was characterised by using ELISA and immunocytochemistry tests. The 50% binding titers were approximately at dilution 10 5and the MAbs reacted with IgM +cells from smears of the head kidney and spleen of the seabass. In both methods, the specific activity was inhibited by using seabass IgM in solution.
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