Abstract
Surface plasmon resonance (SPR) sensor, an optical sensor exploiting special electromagnetic waves-surface plasmon polaritons to probe interactions between an analyte in solution and a molecular recognition element immobilized on the SPR sensor surface, has been widely used in various realms, such as investigating biomolecular interactions and binding properties, detection of biological and chemical analytes, environmental monitoring, food safety and medical diagnostics. This paper reviews the development of SPR sensors and SPR commercial instruments, and emphatically introduces the time-resolved surface plasmon resonance (TR-SPR) techniques. The excellent performances of high sensitivity and rapid detection are easily achieved with TR-SPR spectrometer, whereas the traditional SPR spectrometer cannot be accomplished. Therefore, TR-SPR spectrometer is appropriate for real time analysis of bio-recognition events and small molecular dynamics. However, only two commercial TR-SPR instruments have been exhibited at present, Thermo FT-SPR 100 and Autolab ESPRIT (SPRINGLE). Unfortunately, the high-priced instruments are not suitable for extensive applications in laboratories at present. Herein, a novel commercial TR-SPR spectrometer has been introduced in this review.
Highlights
Over the past decade, surface plasmon resonance (SPR) sensors have attracted a great deal of attention
This paper reviews the development of SPR sensors and SPR commercial instruments, and emphatically introduces the time-resolved surface plasmon resonance (TR-SPR) techniques
With improved experimental design and advanced data analysis methods, high-quality data for the determination of kinetic parameters of molecular interactions phenomena can be obtained. These data promise additional insights into the mechanisms of molecular binding events, which will be important for function-regulatory protein interactions studies in order to unravel the exciting processes in living species. Such a TR-SPR sensor system hold potential for many other applications of paramount importance, including detection of contaminants related to environmental monitoring, human health indicators for medical diagnostics, foodborne pathogens and toxins implicated in food safety and security
Summary
Surface plasmon resonance (SPR) sensors have attracted a great deal of attention. SPR detection method can be used to measure the dynamic adsorption process of the biological molecules on the SPR sensor chip surface in real-time and in situ. SPR method has become a conventional method on the detection of molecular interactions It has been widely used in the field of life science, such as proteomics [1,2], cell signaling [3,4], receptor/ligand [5,6,7,8], antibody/antigen [9,10,11,12,13,14], immune recognition [15,16], cancer research [17,18,19,20] and drug screening [21], etc. SPR method has been used for real-time dynamic research on protein/protein [22], protein/nucleic acid [23], drug molecule/target protein [24] during the molecular interactions. The time resolution is better than or comparable to those of most published sensograms for commonly studied electrochemical and biological reactions [29,30,31,32,33,34]
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