Development and Application of Novel Microfluidic Culture Chips for Germ Cells

Abstract

This program is to improve the traditional germ cell culture technology, and expected to develop and validate the innovative germ cell microfluidic culture technology. The main concept is to use the in vitro microfluidic chip to mimic the fluid growth environment in the living body, and to shape the growth environment of the zygote cells into a bionic concept in the uterus. The main objective is to design to the most suitable growth conditions, the importance of which is to increase the chance of zygote cell culture to maturity and greatly improve the maturity of zygotic cells. Assist Reproductive technology is a related technology for in vitro washing, culturing and storage of human germ cells. The main method is to use an artificial culture medium that mimics the human body fluid of the human reproductive system to perform in vitro washing, culture, and storage of reproductive gametophytic cells. The project will be divided into three parts: 1. Bionic microfluidic chip design and production; 2. Biocompatibility and materialization analysis; 3. Germ cell chip efficacy verification. Therefore, this project will develop a continuous microfluidic chip, using a continuous microfluidic channel to simulate the growth of human germ cells in the fertility tube to the zygotic germ cell growth environment, and hope to improve the artificial reproductive technology success rate and pregnancy rate. Reduce the cost of repeated artificial reproduction, and eventually, develop into medical-grade products and promote local technology to the international market.