Abstract
Molecular markers are powerful tools for analyzing genome diversity within a species, and to evaluate genetic relationships between individuals and populations. Among them, microsatellites (SSRs) are one of the most important polymorphic markers that can be used effectively to distinguish germplasm accessions. These markers present high informative content due to their codominant inheritance, multiallelism, mendelian pattern and good genome coverage. The enrichment methodology for microsatellite development has a superior efficiency in plants, especially when performed using biotin-labeled microsatellite oligoprobes and streptavidin-coated magnetic beads. The development of EST-SSR markers has become a fast and relatively inexpensive way but it is limited to species for which this type of database exists. Given the high polymorphism level of microsatellites when compared to other markers, SSRs have been used to study population structure, for genetic diversity analysis, genetic mapping and marker assisted selection.
Highlights
Hybridization method is very commonly used in crop breeding in order to obtain segregant populations portraying interesting agronomical traits and broad genetic variability
An enriched library was constructed according to Billotte et al (1999) for Instituto Agronômico (IAC)-UNA according to Benchimol et al (2007)
The selection of microsatellite sequences was performed using biotinlabeled microsatellite oligoprobe and streptavidin-coated magnetic beads following a hybridization-based capture methodology adapted from Kijas et al (1994)
Summary
Hybridization method is very commonly used in crop breeding in order to obtain segregant populations portraying interesting agronomical traits and broad genetic variability. This method combines available genes from two or more different genitors in a unique individual (Allard 1999). Molecular markers are powerful tools for analyzing genome diversity within a species, and to evaluate genetic relationships between individuals and populations. Microsatellites or SSRs (Simple Sequence Repeats, Tautz 1989) are one of the most indicated techniques to study polymorphism between DNA sequences These molecular markers are based on PCR reaction that detects loci variations of repetitive sequences. The selective hybridization protocols are very commonly used in microsatellite enrichment (Kijas et al 1994, Billotte et al 1999)
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