Abstract
Soil salinity is a major abiotic stress in Australian lentil-producing areas. It is therefore imperative to identify genetic variation for salt tolerance in order to develop lentil varieties suitable for saline soils. Conventional screening methods include the manual assessment of stress symptoms, which can be very laborious, time-consuming, and error-prone. Recent advances in image-based high-throughput phenotyping (HTP) technologies have provided unparalleled opportunities to screen plants for a range of stresses, such as salt toxicity. The current study describes the development and application of an HTP method for salt toxicity screening in lentils. In a pilot study, six lentil genotypes were evaluated to determine the optimal salt level and the growth stage for distinguishing lentil genotypes using red–green–blue (RGB) images on a LemnaTec Scanalyzer 3D phenomics platform. The optimized protocol was then applied to screen 276 accessions that were also assessed earlier in a conventional phenotypic screen. Detailed phenotypic trait assessments, including plant growth and green/non-green color pixels, were made and correlated to the conventional screen (r = 0.55; p < 0.0001). These findings demonstrated the improved efficacy of an image-based phenotyping approach that is high-throughput, efficient, and better suited to modern breeding programs.
Highlights
Soil salinity is identified as one of the major abiotic stress factors that limits crop productivity across the world [1,2,3]
Medik.) is comparatively more sensitive to salt with more than 90% of yield loss recorded at electrical conductivity (EC) = 3 dS/m (i.e., ~30 mM sodium chloride (NaCl)) compared to other grain crops such as barley (10% yield loss at EC = 6.6 dS/m; ~66 mM NaCl), wheat
This paper describes a novel application of high-throughput phenotyping in a glasshouse-based experiment to quantify salt tolerance in a diverse range of lentil accessions
Summary
Soil salinity is identified as one of the major abiotic stress factors that limits crop productivity across the world [1,2,3]. In Australia, many agricultural regions are affected by a high soil salinity, which can result in poor plant growth due to reduced water and nutrient uptake [4]. Medik.) is comparatively more sensitive to salt with more than 90% of yield loss recorded at electrical conductivity (EC) = 3 dS/m (i.e., ~30 mM sodium chloride (NaCl)) compared to other grain crops such as barley (10% yield loss at EC = 6.6 dS/m; ~66 mM NaCl), wheat The identification of salt-tolerant lentil germplasm is of utmost importance for the sustainability of the lentil industry. The identification of salt-tolerant lentil accessions is dependent
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