Abstract
DNA shuffling is a high-throughput library construction technology that was first described in 1994. Following decades of development, it now has outstanding performance in the reconstruction of DNA, enzymes, and pharmaceutical proteins. The parvovirus adeno- associated virus (AAV) is an ideal gene expression vector because it is non-pathogenic and has potent gene delivery features. However, limitations such as induction of immune response and recalcitrance of some important target cells to AAV infection impede the progress of its clinical study. Using DNA shuffling technology for directed evolution of the AAV capsid protein is expected to resolve these problems. The present paper reviews the technology development and application of DNA shuffling in AAV capsid directed evolution, and discusses the basis of the experiments in this field.
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