Abstract
BackgroundAs the major causative agent of swine viral diarrhea, porcine epidemic diarrhea virus (PEDV) has caused massive losses to the economies of swine raising countries. Accordingly, the serological detection of corresponding antibodies would be beneficial to diagnose PEDV indirectly to control the disease. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant truncated spike (S) protein of PEDV was developed and validated.ResultsThe reaction conditions of the developed indirect ELISA were optimized. This indirect ELISA was compared to indirect immunoinfluscent assay (IFA), and the overall coincidence rate was 96.74% based on testing 368 clinical serum samples with different PEDV antibody levels. No cross-reactivity with other common swine pathogens was detected for the developed S1 indirect ELISA. Finally, the S1 indirect ELISA was applied to detect serum antibodies of 3304 field samples collected from different pig farms in eastern China, and it presented an overall substantial agreement on the PEDV infection status.ConclusionsThis established S1 indirect ELISA is capable of detecting serum antibodies against PEDV, and due to its high sensitivity and specificity, it could be applied for serological evaluation and indirect diagnosis of PEDV infection.
Highlights
As the major causative agent of swine viral diarrhea, porcine epidemic diarrhea virus (PEDV) has caused massive losses to the economies of swine raising countries
Porcine epidemic diarrhea (PED) is a highly contagious swine enteritis caused by porcine epidemic diarrhea virus (PEDV), which belongs to the order Nidovirales and family Coronaviridae
1971, followed by an outbreak of diarrhea in several pig farms in Belgium in 1977 [6]. These outbreaks led to identification of a coronavirus-like particle named CV777, which is recognized as the classic PEDV strain
Summary
As the major causative agent of swine viral diarrhea, porcine epidemic diarrhea virus (PEDV) has caused massive losses to the economies of swine raising countries. Porcine epidemic diarrhea (PED) is a highly contagious swine enteritis caused by porcine epidemic diarrhea virus (PEDV), which belongs to the order Nidovirales and family Coronaviridae. 1971, followed by an outbreak of diarrhea in several pig farms in Belgium in 1977 [6]. These outbreaks led to identification of a coronavirus-like particle named CV777, which is recognized as the classic PEDV strain. PED epidemics have become prevalent in swine-raising countries in Asia, including South Korea, China, Japan, and Vietnam, and can cause enormous economic loss [7, 8]
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