Abstract
The zearalenone (ZEA) monoclonal antibody (mAb) 2D3, one of the highest sensitivity antibodies, was developed. Based on this mAb, it was established of an immunoaffinity column (IAC) coupled with an indirect competitive enzyme-linked immunosorbent assay (icELISA). After optimization, the icELISA allowed an IC50 against ZEA of 0.02 µg L−1. The mAb 2D3 exhibited a high recognition of ZEA (100%) and β-zearalenol (β-ZOL, 88.2%). Its cross-reactivity with α-zearalenol (α-ZOL) and β-zearalanol (β-ZAL) were found to be 4.4% and 4.6%, respectively. The IAC-icELISA method was employed to analyze ZEA contamination in food samples, compared with high-performance liquid chromatography (HPLC). The spiked assay for ZEA demonstrated the considerable recoveries for IAC-icELISA (83–93%) and HPLC (94–108%) methods. Results showed that the mAb 2D3 and IAC-icELISA method posed potential applications in sensitively determination of ZEA in maize.
Highlights
Zearalenone (ZEA) is one common mycotoxin produced by several Fusarium species [1]
The developed IACicELISA method could be potentially extensively utilized in ZEA determination in agricultural products and food-stuffs
Roswell Park Memorial Institute (RPMI) 1640 medium with L-glutamine, penicillin, (+10,000 U mL21) and streptomycin (+10,000 mg mL21), HEPES, and PierceH Rapid enzymelinked immunosorbent assay (ELISA) Mouse monoclonal antibody (mAb) Isotyping Kit were obtained from Thermo-Scientific (Rockford, USA)
Summary
Zearalenone (ZEA) is one common mycotoxin produced by several Fusarium species [1]. ZEA is a resorcylic acid lactone, which usually involved hyperestrogenism and other breeding disorders in pigs, sheep and other farm animals [2]. For the detection of ZEA in foodstuff samples, a number of chromatographic methods are most used, including thin-layer chromatography (TLC) [7], high performance liquid chromatography (HPLC) [8], and liquid chromatography-mass spectrometry (LC-MS) [9] These techniques, are usually high cost and time-consuming. Many immunoassay methods have been developed for rapid detection of ZEA, such as enzymelinked immunosorbent assay (ELISA) [10], fluorescence polarization immunoassay [11], electrochemical microfluidic chips [12], immunochemical test [13], electrochemical magnetic bead-based inmunosensor [14], immunochromatographic strip [15], and so on Among these immunoassay methods, ELISA has been extensively used as a cost- &time-saving, sensitive, quantitative, and high-throughput method. The developed IACicELISA method could be potentially extensively utilized in ZEA determination in agricultural products and food-stuffs
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