Abstract
Abstract An enzyme electrode was developed using the enzyme sulfite oxidase (EC 1.8.3.1) immobilized onto pig intestine. All experimental parameters such as pH, temperature, buffer constituent concentration, etc., were thoroughly investigated and optimized when appropriate. Hydrogen peroxide was monitored amperometrically. The response to sulfite concentration was linear in the range of 5.2 × 10−6 to 1.0×10−3M. The proposed method was found to have a correlation coefficient of 0.952 when evaluated versus the standard titration method.
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