Abstract
Apocynum venetum L. and Eleutherococcus senticosus have been used for hundreds of years to treat hypertension in China. In previous research, there was not a suitable quality control of method for the formulas of Apocynum venetum L. and Eleutherococcus senticosus. It is urgent and essential to develop modern analytical methods for Apocynum venetum L. and Eleutherococcus senticosus to ensure the quality of the formulas. A rapid approach for simultaneous determination of hyperoside, isoquercitrin and eleutheroside E in Apocynum venetum L. and Eleutherococcus senticosus by high-performance liquid chromatography with a diode array detector was described and validated. The full method validation, including the linearity, limits of detection and quantification, precision, repeatability, stability and recovery, was examined. All target components, including isomers of hyperoside and isoquercitrin, were baseline separated in 35 min. The developed method was sensitive, reliable and feasible. With this method, the optimal decoction conditions of Apocynum venetum L. and Eleutherococcus senticosus were selected, and their quality analysis was carried out. Furthermore, an herbal compatibility study of Apocynum venetum L. and Eleutherococcus senticosus based on detecting variations in the content of their active ingredients was performed by the developed HPLC method. It could be an alternative for the quantitative analysis of herbs that contain hyperoside, isoquercitrin or (and) eleutheroside E in the future.
Highlights
Botanical herbs are consumed globally as an essential component of the diet and as medicines or as functional food supplements
Another study reported a microemulsion mobile phase consisting of 2.5% (v/v) n-butanol, 1.2% (v/v) Genapol X-080, 0.5% (v/v) ethyl acetate and 95.8% (w/v) aqueous 20 mM phosphoric acid, and 360 nm was selected to detect six flavonoids of Apocynum venetum L. extract, including hyperoside and isoquercitrin [26]
According to the above literature, water (0.1% (v/v) phosphoric acid) and acetonitrile (0.1% (v/v) phosphoric acid) were selected as the mobile phases in our assay, and 210 nm and 360 nm were both investigated to determine which one was better for detecting hyperoside, isoquercitrin and eleutheroside E simultaneously
Summary
Botanical herbs are consumed globally as an essential component of the diet and as medicines or as functional food supplements. As herbs used in traditional Chinese medicine (TCM), Apocynum venetum L. Et Maxim.) Harms, have been used for hundreds of years to treat hypertension in China. In addition to reducing blood pressure, the formulas can be used to relieve stroke and other heart diseases [1]. Based on the multifold efficacy, an increasing number of patients with hypertension take these herbs. It is urgent and essential to develop modern analytical methods to ensure the quality of the formulas. (Luobuma in Chinese) is a perennial herbaceous or half-shrub plant that grows in central to northwestern China [2].
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