Abstract
Three-dimensional (3D) bioprinting is a revolutionary technology that replicates 3D functional living tissue scaffolds in vitro by controlling the layer-by-layer deposition of biomaterials and enables highly precise positioning of cells. With the development of this technology, more advanced research on the mechanisms of tissue morphogenesis, clinical drug screening, and organ regeneration may be pursued. Because of their self-renewal characteristics and multidirectional differentiation potential, induced pluripotent stem cells (iPSCs) have outstanding advantages in stem cell research and applications. In this review, we discuss the advantages of different bioinks containing human iPSCs that are fabricated by using 3D bioprinting. In particular, we focus on the ability of these bioinks to support iPSCs and promote their proliferation and differentiation. In addition, we summarize the applications of 3D bioprinting with iPSC-containing bioinks and put forward new views on the current research status.
Highlights
The lack of tools for assessing promising drug targets impedes the development of treatments for various conditions, such as spinal cord injury and cardiovascular diseases
Considering that several reviews devoted to 3D bioprinting of Human-induced pluripotent stem cells (hiPSCs) have been published recently, in the present review, we focus on different bioinks prepared by 3D bioprinting and discuss their advantages in supporting and promoting hiPSC proliferation and differentiation
We review the use of hiPSCs in combination with 3D bioprinting for the treatment of neurological, orthopedic, cardiovascular, and hepatic disorders
Summary
The lack of tools for assessing promising drug targets impedes the development of treatments for various conditions, such as spinal cord injury and cardiovascular diseases. Bioprinting permits highly precise and accurate fabrication of biological 3D constructs containing cells, extracellular matrix scaffolds, and biochemical factors Such 3D constructs can better mimic the human in vivo microenvironment than 2D cell culture environments and animal models [3]. In addition to the natural scaffolds, some synthetic components can be designed with tunable mechanical and degradation properties Various adult cells, such as mesenchymal stem cells, chondrocytes, cardiomyocytes (CMs), and endothelial cells, BioMed Research International can be used for bioprinting. Human-induced pluripotent stem cells (hiPSCs) have attracted global attention since their development a decade ago [5] Because of their self-renewal properties and potential for multilineage differentiation, iPSCs may be used to generate a large number of autologous adult cells. In our concluding remarks, we summarize these recent advances and provide an outlook for the future development of these methods
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