Abstract
Objective To establish the experimental animal models of chronic hypoxic pulmonary hypertension (CHPH),sustained hypoxia animal feeding tank with automatic control under theatmospheric pressure was designed and manufactured.Methods ①The tank contained four independent systems--oxygen concentration control system,tank ventilation system,hypoxia alarm system and tank enclosure system.Through software integrated control,this automatic hypoxic animal tank was featured by high degree of automation,simple operation,low cost,and stable system.The oxygen concentration in the tank was stabilized at about 10％ by setting,and the other indicators in it were in accord with national SPF animal husbandry standards.②10 SD rats were randomly divided into the hypoxic group and the normoxic control group (n =5).Those in the hypoxic group had been raised in this automatic animal models tank with set parameters in SPF environment for three weeks,meanwhile,those in normoxic control group were placed in the same SPF animal room and given the same diet.Body weight of rats,the mean right ventricular pressure (MRVP),right ventricular systolic pressure (RVSP),right ventricular hypertrophy index [RV/(LV+ S)] and pulmonary vascular pathological changes were observed.Results Rats in hypoxic group gain weight slowly,and MRVP,RVSP,RV/(LV-S) were significantly higher than those in the control group (P ＜0.01).The lung pathology examination revealed the intrapulmonary vascular wall of rats in hypoxic group was significantly thicker.Conclusions This hypoxia animal feeding tank are in high degree of automation,convenient application,flexible adjustment,simple structure,stable performance,low cost,and easy to operate,which can establish the CHPH rats model well in line with the CHPH pathophysiological changes,and meet the other hypoxic animal experiments needs. Key words: Hypoxic pulmonary hypertension; Animal model; Hypoxia; Rat
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