DEVELOPMENT A VALIDATED HPTLC METHOD FOR QUANTIFICATION OF LINALOOL IN THE OILS AND EXTRACTS OF SAUDI ARABIAN OCIMUM BASILICUM AND LAVANDULA ANGUSTIFOLIA

Abstract

Linalool is an important constituents found in the many Lamiaceae herbs. Ocimum basilicum and Lavandula angustifolia are parts of the several Arabs foods, traditional medicine and homemade formulations. Though, the lack of operative quality control methods for the selection of quality raw materials and formulations having linalool. A validated high performance thin layer chromatography (HPTLC) method was developed and applied for the quantification of linalool in the essential oils and alcoholic extracts of O. basilicum and L. angustifolia from Saudi Arab local markets. Silica gel 60F-254 pre-coated plates (10 × 10 cm²) were selected for the separation and analysis of linalool compound. The mobile phase, n-hexane: ethyl acetate (8: 2, v/v), wavelength at 460 nm, and Rf (0.31) value were used for the separation, detection and quantification of linalool respectively. The developed method was validated for linearity (R² = 0.9988), limit of detection (LOD) (6.99 ng), limit of quantification (LOQ) (14.05 ng), accuracy (% recovery =99.58 ± 0.38), and precision (RSD <1%). The amount of Linalool was found to be 170 µg/g and 2900 µg/g in the alcoholic extract and essential oil of O. basilicum and 120 µg/g and 1400 µg/g of alcoholic extract and essential oil of L. angustifolia respectively. The developed HPTLC method was found to be accurate, sensitive, precise, and suitable for the quantification of linalool in the herbal formulations containing these herbs.