Abstract
The grape is an important horticultural crop that is grown worldwide. Breeding a new grape cultivar by conventional means normally will take several generations of backcross, at least 15 years. The efficiency and speed of selection can be accelerated if genetic markers are available for early screening. This project is designed to generate RAPD markers linked to viticulturally important traits, including seedlessness and pistillate genes. A F1 population with 64 progenies of V. vinifera was used for the RAPD analysis. Bulked Segregant Analysis (BSA) method was used for RAPD primer screening. Three-hundred primers were screened between the two pairs of pooled DNA samples, seeded and seedlessness, pistillate and perfect flowers. At least 10 primers produced one polymorphism each between the pools. Further analysis revealed that one of these RAPDs cosegregated tightly with the seedlessness trait, while the others either had loose linkage or no linkage to the traits. To make the RAPD marker useful for breeding selection, an attempt was made to convert it into SCAR marker. The results demonstrated that the RAPD marker may be useful for grape breeding and interpreting inheritance of a particular trait in grapes.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
