Abstract
We present a biosensor based on the transport protein lactose permease (LP) which is imbedded in a supported planar lipid bilayer (SPB) membrane, and which allows the potentiometric detection of lactose via pH measurement. Different experimental setups were used to study systematically the electric properties of the different biosensor components. In a first step the preparation of the SPB is investigated by means of high-frequency capacitance—voltage measurements. The sensor consists of an ion-sensitive field-effect transistor (ISFET) coated with the SPB membrane, the latter containing the lactose/H + cotransporter LP from Escherichia coli ( E. coli). The sensor effect consists of a rise in the proton concentration in the small volume between the lipid membrane and the gate area upon addition of lactose to the external medium. This effect is monitored directly by the pH-sensitive ISFET.
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