Developing a human mesenchymal stem cell culture medium with high growth efficient, 3d culture supported, serum-free and xeno-free properties.

Abstract

Background & Aim Human mesenchymal stem cell (MSC) therapies have been tested clinically for a variety of disorders, including Crohn's disease, multiple sclerosis, graft-versus-host disease (GVHD), bone fractures, sepsis, and cartilage defects. Despite of the remarkable clinical advancements in this field, most cell manufacturing processes still rely on the traditional culture medium containing fetal bovine serum (FBS). The highly inconsistence nature and xenogenic infection risks of the bovine serum remain a challenge for the standardization and massive production for clinics. Here, we successfully develop a medium, featured with serum-free, xeno-free components and high efficacy for MSC growth, named as AllPhase. Methods, Results & Conclusion The medium on human adipose tissue-derived mesenchymal stem cells (ADSC) and Wharton's jelly mesenchymal stem cells (WJ-MSC) enables competitive expansion performance, comparing to traditional culture medium and can maintains tri-lineage mesoderm differentiation potential after fifth passages. Furthermore, most commercial serum-free media need expansive ECM coating, like fibronectin or other commercial coating materials, to support cell adhesion. Especially, AllPhase can support ADSC expansion under 3D culture condition without microcarriers. We hope that our medium can support most bioreactors and cell industrial production for MSC clinical applications.