Abstract
A fast and simple purification method for withaferin A (WFA) in ashwagandha extract was successfully developed using a preparative column packed with superficially porous particles (SPPs). The SPP column provided better resolution and a 45% decrease in run time over a traditional preparative column packed with totally porous particles. The performance benefits of SPP preparative columns, especially at high flow rates, align well with the needs of pharmaceutical discovery laboratories and other high-throughput environments.
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