Developed ratiometric fluorescent probe as a logic platform for potential diagnosis of thyroid disease and diabetes and fluorescent ink

Abstract

Iodine and glucose are indispensable substances whose abnormal levels are closely associated with a variety of diseases. Herein, CDs and RhB embedded Zr-metal-organic framework (RhB-CDs@MOF-808) as dual-emitting ratiometric fluorescent probe was fabricated via post-synthetic modification (PSM). The constructed ratiometric probe presents the emission at 450 nm of CDs was served as a reference to calibrate the emission at 575 nm attributed to RhB to achieve a remarkable self-calibration function. The fluorescence of probe is quenched by I3− by virtue of inner filter effect (IFE), and the I3− is reduced by glucose to achieve the recovery of fluorescence. In view of this, an analytical platform for the continuous detection of I3− and glucose was established with wide linear ranges (I3−: 0–220 μM; glucose: 0–180 μM) and low LODs (I3− : 0.031 μM; glucose: 0.026 μM). The probe has been successfully exploited to the determination of iodine and glucose in real samples of food andbiology. More interestingly, an INHIBIT logic gate was established to provides a new platform for the preliminary diagnosis of thyroid disease and diabetes in clinical medicine. The probe as fluorescent ink achieved the continuous detection of iodine and glucose on a test paper expanding its potential application in samples containing iodine and glucose. This is the unprecedented attempt to exploit a ratiometric fluorescent probe for continuous detection of iodine and glucose, which can provide a new approach for the development of low-cost, sensitive and credible biological and clinical diagnostic thyroid disease and diabetes.