Develop of lateral flow immunochromatographic test and PCR for detection of salmonella enteritidis in poultry farm

Abstract

Background: Avian salmonellosis continue to cause economic losses, particularly in those parts of the world where the poultry industries are continuing to intensify and where open sided housing is common. Salmonellae are responsible for considerable losses in the poultry industry through the death of birds and loss in production Methods and materials: Two diagnostic tools were developed and compared to detect SE in poultry farms. Lateral flow immunochromatographic test (LFIT) was considered a field and laboratory test while PCR was absolutely laboratory test. About 100 samples were tested by the two developing methods and the results were compared with bacteriological methods Results: The minimal bacterial count that's give positive of LFIT and PCR was 102 CFU/0.1 ml and 10 CFU/0.1 ml respectively. For LFIT we could increase the sensitivity results to 1CFU/0.1 ml by pre incubating of the sample in trypticase soya broth at 37 °C for 6 h. The sensitivity, specificity and accuracy of LFIT as compared to bacteriological examination were calculated and were found to be 91%, 80% and 90% respectively for PCR was 98.8%, 82.3% and 96% respectively. Conclusion: The LFIT are not only very rapid (5 m) but also are simple, convenient, has long shelf time and can be used by untrained personal at poultry farm site without requirement of additional equipment like as PCR or bacteriological examination. Moreover the technology has been strongly improved, which will be reflected on its sensitivity and specificity. These tools are badly required for routine diagnosis in the laboratory and under field conditions.