Abstract
Solid state NMR has been tremendously useful in characterizing the structure and dynamics of model membranes composed of simple lipid mixtures. Model lipid studies employing solid state NMR have included important work revealing how membrane bilayer structure and dynamics are affected by molecules such as antimicrobial peptides (AMPs). However, solid state NMR need not be applied only to model membranes, but can also be used with living, intact cells. NMR of whole cells holds promise for helping resolve some unsolved mysteries about how bacteria interact with AMPs. This mini-review will focus on recent studies using 2H NMR to study how treatment with AMPs affect membranes in intact bacteria.
Highlights
Model lipid studies employing solid state NMR have included important work revealing how membrane bilayer structure and dynamics are affected by molecules such as antimicrobial peptides (AMPs)
Much attention has been given to the mechanisms by which AMPs disrupt the membrane bilayers of bacterial cells, permeabilizing them and dissipating the membrane potential [1,2,3]
On the one hand, function is often studied via minimal inhibitory concentration (MIC) assays with bacteria, which indicate the minimum concentration of AMP needed to prevent bacterial growth [9,10,11]
Summary
Sanford Burnham Prebys Medical Discovery Institute, United States. Specialty section: This article was submitted to Pharmaceutical Innovation, a section of the journal
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