Abstract
[ 2H]Chondroitin sulfate was prepared by partial N -deacetylation of chondroitin sulfate ( via hydrazinolysis) followed by treatment with [ 2H 6]acetic anhydride. 2H NMR spectra of [ 2H]chondroitin sulfate in the presence of human plasma low density lipoprotein provide evidence for a soluble complex stoichiometry of 3 (and possibly 2) lipoproteins per polysaccharide molecule, and allow a rough estimation of the dissociation constant K d.
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