Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats.

Tetiana Halenova,Tatiana Pleteneva,Anton Syroeshkin,Tatiana Maximova,Igor Zlatskiy

doi:10.3390/molecules25010023

Abstract

In this study, we present the potential application of deuterium-depleted water (DDW) for the prevention and adjuvant treatment of obesity in rats. We tested the hypothesis that DDW can alleviate diet-induced obesity (DIO) and its associated metabolic impairments. Rats fed a high-fat diet had an increased body weight index (BWI), glucose concentration, and level of certain proinflammatory cytokines; decreased levels of insulin in the serum; decreased tryptophan and serotonin in the brain, and a decreased concentration of some heavy metals in the liver. Drinking DDW at a concentration of 10 ppm deuterium/protium (D/H) ad libitum for 3 weeks restored the BWI, glucose (serum), tryptophan (brain), and serotonin (brain) levels and concentration of Zn in the liver in the DIO animals to those of the controls. The levels of proinflammatory cytokines (IL-1β, IL-6, IFNγ) and anti-inflammatory TNFα were decreased in DIO rats, while anti-inflammatory cytokine (IL-4, IL-10) levels remained at the control levels, which is indicative of a pathophysiological syndrome. In contrast, in groups of rats treated with DDW, a significant increase in anti-inflammatory (IL-4, IL-10) and proinflammatory cytokines (IFNγ) was observed. This finding indicates a reduction in systemic inflammation in obese animals treated with DDW. Similarly, the high-fat diet caused an increased level of oxidative stress products, which was accompanied by decreased activity of both superoxide dismutase and catalase, whereas the administration of DDW decreased the level of oxidative stress and enhanced antioxidant enzyme activities.

Highlights

Obesity is one of the major problems of global significance [1]
The initial weight of the diet-induced obesity (DIO) + MilliQ rats increased by 45% for the first 5 weeks of the experiment
We found that the depleted water (DDW)-normalized serotonin and tryptophan levels in the brain impaired by DIO (Table 1)

Summary

Introduction

Obesity is one of the major problems of global significance [1]. This pathological condition is strongly associated with multiple comorbidities, including different metabolic complications that can affect almost every human organ system. Obesity is a major risk factor for insulin resistance [2], diabetes [3], cardiovascular disease [4] and certain forms of cancer [5]. Excessive fat accumulation in the body can lead to pathological changes in respiratory and musculoskeletal physiology [6]. There is a lack of effective therapeutic approaches for the treatment of obesity and prevention of its related complications. There has been increased interest in the use of dietary supplements and pharmaceuticals for obesity treatment.

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Discussion

Conclusion