Deuterium and tritium incorporation into 5-Oxo-Pro-His-Pro-NH2 by isotope exchange

Abstract

When metal catalysts of the same group are used, the nature of the inorganic support with the surface-deposited peptide becomes the crucial factor determining the yield and hydrogen isotope content of 5-oxo-Pro-His-Pro-NH2. The label distribution in the amino acid residues of 5-oxo-Pro-His-Pro-NH2 was found. The label was shown to be mainly incorporated in His. The synthesis in the presence of deuterium or tritium afforded labeled specimens containing about 3 deuterium atoms or 2.5 tritium atoms (about 70 Ci/mmol) per peptide molecule.