Detrimental effects of cerium oxide nanoparticles on testis, sperm parameters quality, and in vitro fertilization in mice: An experimental study.

Abstract

BackgroundCerium oxide nanoparticles (CeO NPs) as an important nanomaterial have a wide range of applications in many fields and human beings' exposure to this nanomaterial is unavoidable. The effects of CeO NPs on the male reproductive system are controversial.ObjectiveTo determine the effects of the administration of CeO NPs on the testis tissue, sperm parameters, and in vitro fertilization (IVF) in mice.Materials and MethodsTwenty-four male mice were divided into three groups (n = 8/each): one control and two experimental groups receiving CeO NPs at doses of 50 and 100 mg/kg body weight, respectively, for 35 days. At the end of the experiment, the diameter of seminiferous tubules (SNTs), epithelial height of SNTs, spermiogenesis index in testes, sperm parameters (count, motility, viability, and morphology), sperm chromatin condensation, DNA integrity, and IVF assays were analyzed.ResultsHistological results showed that the tubular diameter, the epithelial height of the SNTs, and the spermiogenesis index were significantly decreased in the experimental groups receiving CeO NPs. All sperm parameters in the experimental groups were significantly reduced and, additionally, the percentages of immature sperms and sperms with DNA damage were significantly increased in groups treated with CeO NPs compared to the control. Furthermore, the rates of IVF and in vitro embryo development were decreased.ConclusionCollectively, the current study showed that oral administration of CeO NPs in mice had detrimental effects on the male reproductive system through inducing testicular tissue alterations, decreasing sperm parameters quality, and also diminishing the IVF rate and in vitro embryonic development.