Detoxification mechanisms of biochar on plants in chromium contaminated soil: Chromium chemical forms and subcellular distribution

Abstract

The complete pathway of chromium (Cr) transfer from soil to plant tissues and subcellular components under biochar amendment remains to be quantified, as well as the involved diverse detoxification processes in roots and stems respectively. Pot experiments and quantitative analysis were conducted to investigate Cr fixation in soil amended with Enteromorpha prolifera-derived biochar and subsequent phytoprocesses (Cr uptake, transfer, and phytotoxicity) in cultivated Secale cereale L. (rye). The results indicated that adding 5–30 g kg−1 of biochar increased the residual form of Cr (B4) in soil by 8–21% and decreased the bioavailable form of Cr (B1) by 9–29%. For Cr transferred to rye, Cr in the rye was mainly present in the low-toxicity bound state, with the acetic acid-extracted Cr (F4) (45–54%) in roots and the NaCl-extracted Cr (F3) (37–47%) in stems. The subcellular distribution of Cr in both roots and stems was predominantly in the cell wall and residues (T1), followed by the cytoplasm (T4). Partial least squares path model (PLS-PM) was used for quantifying the effect of biochar on the form changes and subcellular detoxification of Cr from soil to roots and stems to sub-cells. In soils, biochar reduced the bioavailability of Cr and decreased the transfer of Cr to rye. In plant roots, Cr was distributed mainly as low-toxicity phosphate complexes in cell walls and vacuoles in sub-cells (with the largest path coefficients of 0.90 and −0.91, respectively). In the stems, Cr was distributed mainly as proteins integrated into the cell walls and vacuoles. This was due to the difference in subcellular compartmentalization of detoxification in the roots and stems. These PLS-PM results provide new insights into the entire process of pollutant detoxification in complex environments.