Detoxification mechanisms, defense responses, and toxicity threshold in the earthworm Eisenia foetida exposed to ciprofloxacin-polluted soils

Abstract

The widespread application of antibiotics poses health risks for agro-ecosystems. This study examined the effects of ciproflaxin (CIP)-polluted soils (0–51.2mgCIP/kg) on the earthworm Eisenia foetida. The enhanced activities and isozyme levels of superoxide dismutase (SOD) and ascorbate peroxidase after 15days of CIP exposure suggested reactive oxygen species overproduction and thus the generation of oxidatively damaged proteins (e.g., carbonylated proteins) in the earthworms. Under mild CIP stress, the 20S proteasome was capable of degrading most of the damaged proteins independent of ubiquitin. Under severe stress, proteases and endoproteases were up-regulated and maintained the proteolysis as 20S proteasome activity diminished. These observations suggested that, together with glutathione S-transferases, which also participated in the detoxification, 20S proteasome, proteases, endoproteases, and antioxidant enzymes constituted a detoxification and defense system in the earthworms. The biphasic dose responses of these cellular components confirmed that the dose range tested was reasonable for the bioassay of CIP-polluted soils. Our results also demonstrated the potential utility of SOD and ubiquitin as highly sensitive biomarkers in the early bioassay of CIP-polluted soils. Bases on the results, a toxicity threshold for CIP-polluted soils of 3.2–6.4mgCIP/kg soil can be proposed.