Abstract
Hepatitis is a metabolic system disease which is a serious challenge to the medical and healthcare system of the world. This study attempted to investigate the therapeutic effect and illustrate the regulation pharmacological mechanism of Detoxification II Prescription on ACLF. In this study, the rats were injected with D-galactosamine to establish ACLF-rat models, and the levels of cholinesterase (CHE), alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), and total bilirubin (TBiL) were measured with the related kits to reflect the liver functions of the rats. The levels of IL-17, IL-6, and IFN-γ in the serums of the rats were detected by qRT-PCR, and the percentages of Th-17 cells in CD4+ cells of the rats were measured by flow cytometry assay. In the results, the increased ALT, AST, TBiL, IL-6, IL-17, IFN-γ, and percentage of Th-17 cells in CD4+ and decreased ALB and CHE were found in the serums of the ACLF-rats, while Detoxification II Prescription could partly reverse those indexes of the ACLF-rats. Moreover, it was also found that Detoxification II Prescription could inhibit the expression of P38MAPK, and P38MAPK downregulation obviously improved the liver function indexes of the ACLF-rats including the levels of ALT, AST, TBiL, IL-6, IL-17, IFN-γ, and percentage of Th-17 cells in CD4+ cells. In conclusion, this study suggested that Detoxification II Prescription could suppress the Th-17/IL-17 inflammatory axis to improve the liver function of ACLF-rats via inhibiting the activity of the P38MAPK pathway.
Highlights
Hepatitis is a metabolic system disease which is a serious challenge to the medical and healthcare system of the world. is study attempted to investigate the therapeutic effect and illustrate the regulation pharmacological mechanism of Detoxification II Prescription on acute-on-chronic liver failure (ACLF)
ACLF-rats are always used for the research of acute-onchronic liver failure [17]. e levels of ALT, AST, total bilirubin (TBiL), ALB, and CHE have been widely used as the physiological indexes for the diagnosis of the liver-related diseases in clinic [18]
To investigate the effect of Detoxification II Prescription on ACLF, the ACLF models were treated with GaIN to establish the ACLF-rats, and it was found that the levels of ALT, AST, and TBiL in the serums of the ACLF-rats increased, while the levels of ALB and CHE significantly decreased compared with normal rats
Summary
Hepatitis is a metabolic system disease which is a serious challenge to the medical and healthcare system of the world. is study attempted to investigate the therapeutic effect and illustrate the regulation pharmacological mechanism of Detoxification II Prescription on ACLF. It was found that Detoxification II Prescription could inhibit the expression of P38MAPK, and P38MAPK downregulation obviously improved the liver function indexes of the ACLF-rats including the levels of ALT, AST, TBiL, IL-6, IL-17, IFN-c, and percentage of -17 cells in CD4+ cells. Detoxification II Prescription is one of the traditional Chinese medical strategies for hepatitis treatment and consists capillary artemisia, spreading hedyotis herb, and rhubarb, and those herbs have been confirmed to have some functions in anti-inflammation and improving liver functions [13, 14]. Is study attempted to explore the function of Detoxification II Prescription on ACLF and illustrate the regulation mechanism of this traditional Chinese medicine on the inflammation of the liver
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