Abstract
To evaluate the efficacy of bovine amniotic membrane homogenate (BAMH) on wounded ex vivo rabbit corneas. Eighteen corneas obtained from normal rabbit eyes were wounded equally using a 6mm trephine and cultured into an air-liquid interface model. Corneas were treated with phosphate-buffered saline (PBS) (n=6, control group), 0.2% ethylenediaminetetraacetic acid (EDTA; n=6), or BAMH (n=6). All treatments were applied topically 6times/day. Each cornea was macrophotographed daily with and without fluorescein stain to assess epithelialization and haziness. After 7days, corneal transparency was evaluated, and the tissues prepared for histologic analysis of viability, and total and epithelial thickness. The mean epithelialization time was 6.2±0.82days for the control group, 6.2±0.75days for the EDTA-treated group, and 5.1±0.40days for the BAMH-treated group, demonstrating a significant difference between the BAMH and the other groups. The corneas that received EDTA had better transparency compared with the other groups. Histologically, all corneas had adequate morphology and architecture after healing. Analysis of corneal and epithelial thickness revealed no significant difference among groups. Bovine amniotic membrane homogenate is an effective and promising treatment for stromal and epithelial ulcers.
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