Abstract

This research was aimed to isolation and identification of Aspergillus niger from different location of garden in Tikrit university, to used in Se-NPs synthesis alone and conjugated with Zn, then its characterizes by detected the spectroscopy, shape and size of the nanoparticles, also determine the efficacy each of Se-NPs or Zn alone or after conjugated when orally dosage to laboratory rats that’s induced immunosuppressed by sandimmune on IgA, IgG and IL-6. The results were found that ability to isolated and identified the Aspergillus niger from different sites in the gardens of the Tikrit University. The synthesis of Se-NPs were obtained by converting sodium selenite (Na2SeO4) used the Aspergillus niger extract, also they obtained the Se-NPs conjugated with Zn. The confirm of synthesis were inferred through the color was changed to oranges. The value of these particles alone or their genera appeared at a wavelength of 262 and 269 nm, respectively. The scanning electron microscope (SEM) showed spherical particle shapes and sizes at 52.90 nm which were confirmed by X-ray diffraction. The orally dosage of Se-NPs for male laboratory rats alone or in combination induced immunusupressed affected the renormalization of IgA, IgG, and IL-6 t. Their normal levels of IgA at 205, 215 and 242 (mg/dL) and the IgG at 1019, 1046 and 1346 (mg/dL) while of IL-6 at 3.60, 4.13 and 6.63 (pmol/mL) respectively. These effect were refered the parameters to became equalized with their values in the group of control animals and returned to normal.

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