Determining the effect of hydration upon the properties of ligaments using pseudo Gaussian stress stimuli

Abstract

The level of tissue hydration is known to effect viscoelastic material properties. However, prior studies have not fully investigated the effect of hydration on dynamic behavior nor compared the results of transient and dynamic behavior. The material properties of medial collateral rat knee ligaments were studied in relation to hydration, using (sequentially) 0.3 osmolar artificial interstitial fluid (AIF), solutions of AIF plus sucrose with osmolarity 1.05, 1.80 or 2.55, and then AIF. In each solution, the complex compliance was determined as a function of frequency, and the creep response was measured. Complex compliance was determined from a constitutive model created by applying a 0.4±0.2 MPa pseudo Gaussian (PGN) stress stimulus to the ligament. Dehydration caused a reduction in cross-sectional area that was linearly related to the osmolarity of the solution. Reductions of up to 52% were observed and were reversible upon rehydration. Dehydration caused a reduction in the creep rate that was not immediately recovered upon rehydration. The storage compliance was reduced by up to 50% with dehydration; these changes were reversed upon rehydration. The loss compliance and phase angle were not affected by dehydration. Transient and dynamic experiments examine different viscoelastic characteristics and both types of tests appear to be necessary to fully characterize the effects of hydration.