Determining the best graft‐sealant combination for skull base repair using a soft tissue in vitro porcine model

Abstract

The purpose of this work was to compare the absolute breaking strength of various soft tissue skull base (SB) repairs in an in vitro porcine model. A burst pressure (BP) testing system was designed using an axial loading force to create increasing hydraulic pressure. Defects measuring 0.5 × 0.5 cm were created in fascia lata samples. Defects were repaired using various grafts (pericranium and 2 different dural substitutes, Alloderm(®) and Durasis(®)) measuring 1.0 × 1.0 cm to cover the deficient area. Grafts were further reinforced onto the fascia background with either fibrin glue (Tisseel(®)) or hydrogel sealant (DuraSeal™). Each combination of graft and sealant was conducted 6 times and tested 24 hours after the repair. The mean BP (±standard deviation [SD]) were as follows: DuraSeal™-Alloderm, 12.5 ± 5.8 mmHg; DuraSeal™-Durasis, 21.8 ± 20.7 mmHg; DuraSeal™-pericranium, 44.7 ± 30.1 mmHg; Tisseel-Alloderm, 30.6 ± 26.3 mmHg; Tisseel-Durasis, 15.8 ± 18.6 mmHg; and Tisseel-pericranium, 95.5 ± 86 mmHg. One-way analysis of variance showed that the strongest type of repair was Tisseel-pericranium when adjusting for the others (p < 0.0001). The difference in mean BP of repair with DuraSeal™ vs Tisseel(®) was not statistically significant (p = 0.22). Comparing sealants, the use of Alloderm(®) or Durasis(®) decreased the strength of the repair in comparison to pericranium (p < 0.0001). Bonferroni analysis showed a significant difference between pericranium and Alloderm(®) (p < 0.05) and between pericranium and Durasis(®) (p < 0.05) but not between Alloderm(®) and Durasis(®) (p > 0.05). In this model, the strongest type of repair (pressure 6 times higher than normal intracranial pressure) was the combination of Tisseel(®)-pericranium. Our data will help guide surgeons who repair SB defects to choose the best graft and sealant.