Determining the bacterial cell biology of Planctomycetes

Christian Boedeker,Patrick Rast,Susanne Engelmann,Harald Engelhardt,Daniela Borchert,Greta Reintjes,Margarete Schüler,Roberto Kolter,Olga Jeske,Martin Kucklick,Laura Van Niftrik,Manfred Rohde,Damien P Devos,Christian Jogler,Mareike Jogler,Miroslava Schaffer,Rudolf Amann,Muriel C F Van Teeseling

doi:10.1038/ncomms14853

Abstract

Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms.

Highlights

Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake
If such membrane invaginations were smaller in diameter than the diffraction limit of light, they would be visible as red dots in wide field (WF) microscopy
Since genetic tools exist for P. limnophila[25] but not for G. obscuriglobus, we explored the potential role of membrane coat-like proteins (MCs), expected to function in planctomycetal endocytosis, in P. limnophila

Summary

Introduction

Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. Planctomycetal vesicles have been suggested to support endocytosis-like uptake of macromolecules into the paryphoplasm of the planctomycetal species Gemmata obscuriglobus[15] This would be the only vesicle-based uptake system to be found outside of the eukaryotic domain[16,17]. Research on planctomycetal cell biology is hampered by a paucity of genetic tools[25,26]

Methods

Results

Conclusion