Abstract
The aim of this work was to measure N-acetylneuraminic acid (Neu5Ac) in milk-based infant formulas. The analysis was performed by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). The total Neu5Ac were released using trichloroacetic acid and hydrochloric acid and purified using a HLB column. The linearity from 0.05 to 5.0 μg/mg Neu5Ac was adequate. Sialic acid recoveries ranged from 91.8% to 112.4%. The detection and quantification limits (limit of detection, 0.01 μg Neu5Ac/mg; limit of quantitation, 1.08 μg Neu5Ac/mg) were low enough to determine the sialic acid in infant formulas. The validated method is highly reproducible and sensitive, and it is easy to perform.
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