Determining Lipophilic Pyrethroids and Chlorinated Hydrocarbons in Fortified Ground Beef Using Ion-Trap Mass Spectrometry

Abstract

A rapid procedure has been developed that is offered as an improvement for the analysis of lipophilic insecticides in meat. The number of physical and chemical manipulations required of previous methodologies to separate pesticide analytes from fat has been minimized, and the number of solvent evaporations has been reduced to one. Removal of fat from the meat extracts was accomplished with an iso-octane/acetonitrile partition and Florisil column cleanup. Recoveries of the organochlorine pesticides, lindane, p,p‘-DDE, p,p‘-DDT, heptachlor epoxide, and dieldrin, and the pyrethroids, bifenthrin, permethrin, cypermethrin, fluvalinate, and deltamethrin, from fortified meat samples ranged from 59 to 82% with typical relative standard deviations of 10% except for p,p‘-DDT, which gave lower, more variable recoveries likely due to degradation. Limits of detection of the pesticides in ground beef ranged from 2 ng/g for bifenthrin and p,p‘-DDE to 24 ng/g for heptachlor epoxide. Keywords: Pesticide residue analysis; ...