Determining diets for fishes (Actinopterygii) from a small interior British Columbia, Canada stream: a comparison of morphological and molecular approaches

Abstract

AbstractAnalysis of food webs is important for defining functional components of ecosystems, but dietary data are often difficult to obtain and coarsely characterised. We compared three methods of rainbow trout (Oncorhynchus mykiss(Walbaum); Salmoniformes: Salmonidae) and prickly sculpin (Cottus asperRichardson; Scorpaeniformes: Cottidae) gut content analysis: traditional morphological taxonomy of prey items, genetic sequencing of individual prey items, and next-generation sequencing of homogenised gut contents. Prey analysis of invertebrates by morphological identification allowed order-level classifications and produced ecologically important count and mass data. Sequencing individual specimens provided greater taxonomic resolution, while next-generation sequencing of stomach contents revealed more prey diversity in the diets of both fish species as it was possible to detect prey that were degraded beyond visual recognition. Both fish species exhibited generalist feeding characteristics; however, terrestrial Insecta were a large diet component for rainbow trout. This study demonstrates an efficient approach for prey analysis using molecular techniques that complement traditional taxonomy.