DETERMINE THE EFFICACY OF CARBOPLATIN WITH VITAMIN C IN DALTON'S LYMPHOMA TUMOR IN MICE MODEL

Abstract

Carboplatin, a plat inum - containing anticancer drug treatment cau sed a significant increase in the life span of ascites Dalton’s lymphoma tumor bearing mice. However , as compared to carboplatin alone, combination treatment with vitamin C plus carboplatin resulted in better therapeutic efficacy against Dalton's lymphoma tumor. To investigate difference in resistance mechanism to d i a m i n e ( cyclobutane - 1,1 - di carboxylato (2 - ) - O,O’) platinum (CBDCA) and changes in antioxidant levels in liver tissues in relation to different stages of Dalton’s lymphoma tumor growth and the fol lowing carboplatin treatment. The reduction in intracellular accumulation of CBDCA is thought to be an important factor indicating that CBDCA resistant in DLA cell are mutually cross - resistant. Glutathione level increased in liver of tumor bearing compared to normal mice. I ntracellular accumulation of platinum found to be higher in tissue, after carboplatin treatment the platinum accumulation were significantly decre a sed . Antioxidant defense systems such as superoxide dismutase , glutathione peroxidase gluta thione reductase activities and Glutathione – S - transferase activity w ere determined in tissues of carboplatin treatment at different stages of Dalton’s lymphoma tumor gr owth . Resistance mechanism based on reduction of intracellular drug accumulations and i ncreased activity of GST also depend the level of antioxidant enzymes , GST activities are playing significant role in development of resistance in the presences of tumor under various newly developed platinum derivative factor. Anticancer drugs kill suscep tible cells through induction of apoptosis. Alteration of apoptotic pathways in drug –