Abstract
The intention of the present study was to assess the effect of fresh natural food on spermatophore quality and analysis by the way of sperm weight, count, viability as well as the proximate analysis of offered food and shrimp brood stock. The experiment was carried out with the three following treatments; fresh squid, polychaete and beef liver up to 5 weeks. All parameters were measured by the starting of 3 rd week (15 days) and the end of the (35 days) of the experiment. Spermatophore quality was evaluated by spermatophore weight, sperm count, viability and proximate analysis of the treatment and shrimps. The content of three essential fatty acids, Arachidonic acid (AA), Eicosapentanoic acid (EPA) and Docosahexaenoic acid (DHA) from wild Penaeus monodon brood stock were evaluated in comparison with natural diet fed to P. monodon . Spermatophore of wild male brood stock contained higher levels of AA than those of artificial fed males. Polychaete has higher proportion of AA to EPA and DHA. At the end of the treatment the sperm viability and count, shrimp and spermatophore weight were significantly different among treatment and control (p<0.05). The present study concludes that the fresh squid diet is highly preferred over the diets influence on increasing the spermatophore quality, therefore use of fresh squid only is recommended for the maturation of male P. monodon brood stock. // //
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