Determination the therapeutiction of Datura metel leaves extract for some urinary system bacteria in rabbits (in vitro and in vivo )

Abstract

This study was designed to evaluate the effects the watery and alcoholic extracts from leaves of Datura metel in vitro to ten of pathogenic bacteria and study the watery extract toward Candida on the urinary system in rabbits. The results showed the watery and alcoholic extracts from leaves of Datura metel have antibacterial activity against both Gram-positive (Staphylococcus. aureus – Streptococcus .agylactiae ) And Gram-negative bacteria (Klebsiella. pneumonia –Proteus. vulgarities – E.coli – Pseudomonas – Vibrio – Salmonella – Enterobacter ) and Candida. albicans, however, the watery extract of Datura metel were more potent than alcoholic extract against pathogenic bacteria (80%) . According to results , Candida pathogen that more sensitive toward Datura metel watery extract was choosing to injected intraperitoneally as experimental infection in laboratory animals (vivo) which cause morphological and histopathological degenerative lesion of kidney cortex and medulla tissue in addition to change of renal profile test that include blood urea nitrogen, creatinine, creatinine kinase, uric acid, in addition to Potassium. but after watery extract of Datura metel injected in these laboratory animals cause significant improvement (p≤0.01) in the value of blood urea nitrogen, creatinine, creatinine kinase, and uric acid. Potassium concentration, histopathological studies confirm these results which include regeneration of degenerative lesion for medulla and kidney cortex with convoluted tubules tissue.