Abstract
Summary1. An alternative method for the extraction and quantitation of free and conjugated corticosteroids in human plasma has been described. 2. With the use of the relatively pure monoglucuronide of tetrahy-dro-E, it has been possible to show that the recovery of conjugated corticosteroids is satisfactory within a broad range of concentrations. 3. Following the administration of large oral doses of free tetrahydro-E, very high levels of conjugated corticosteroids, in excess of 3 mg/100 ml of plasma, have resulted. The material has been definitively identified as tetrahydro-E. A second component, possibly the 20-reduced derivative has been detected. 4. The renal clearances of the free and conjugated steroids in human plasma have been determined.
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