Abstract

A rapid method for the colorimetric determination of amylase activity in serum and urine is described. This method uses amylose as substrate; it is very simple to perform, uses only 0.4 ml of serum, and can be completed in approximately 25 minutes. Preparation of amylose and a criterion of purity of the product are given. The influence of various pertinent factors has been examined and is discussed. Application of the method to 100 cases and a working ·normal” range are given.

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