Abstract
Addition of an equal volume of 20% ( w w ) polyethylene glycol 6000 to human serum leads to separation of two major zinc ligands. α 2-Macroglobulin, a zinc metalloprotein, is precipitated, while albumin, which forms a zinc-protein complex in serum, remains soluble. Total serum zinc and albumin-bound zinc are measured; α 2-macroglobulin bound zinc is obtained by difference. The following values of zinc distribution were obtained in a study of 16 normal males; serum zinc, 82 ± 9 (mean ± standard deviation) μg/100 ml; albumin-bound zinc 66 ± 7 μg/100 ml and α 2-macroglobulin-bound zinc 16 ± 3 μg/100 ml.
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