Abstract
Abstract For a given reversed phase HPLC system the zero retention time (t0) can be determined by measuring brutto retention times at different temperatures. For calculation of t0 value temperature intervals must be equidistant in 1/T. If at least two compounds are separated on the column having almost the same sorption enthalpies a more simple intersecting point method can be practised. In this case only two temperature points are necessary for graphic evaluation. The presented methods for determination of t0 values are proved for noradrenaline and adrenaline under selected experimental conditions using aqueous solutions of alkaline perchlorates as mobile phases. Thus t0 values can be determined with an accuracy and a precision of less than ± 3%.
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