DETERMINATION OF ZEARALENONE BY THE CAPILLARY ZONE ELECTROPHORESIS-UV DETECTION AND ITS APPLICATION TO POULTRY FEED AND CEREALS

Abstract

A capillary zone electrophoretic method for the determination of zearalenone is described in this study. In the separation, a run buffer consisting of 20 mM sodium tetraborate at pH 9.0 with 15% acetonitrile applying 15 kV, injecting 10 s at 50 mbar was utilized. Phenobarbital was a suitable internal standard and the signals were recorded at 254 nm. Zearalanon and internal standard appeared at 5.46 min (RSD% 0.20) and 6.35 min (RSD% 0.18), respectively. The repeatability results are in the range of 0.01–1.58 for inter-day. The linearity was investigated in the range of 5.20 × 10−7 M to 7.86 × 10−5 M ZEA and it was linear fitting to the equation of [rPN = 244803.8 C(M) −0.0348; r = 0.9999]. The LOD and LOQ were calculated to be 2.70 × 10−8 M (8.25 µg/L) and 8.17 × 10−8 M (25 µg/L), respectively. Then, the method was successfully applied to the analysis of ZEA in poultry feeds, flour of maize, grain, and certain cereal samples such as fibrous biscuit, popcorn, and rice crisp.