Abstract
A method for the determination of water-soluble vitamins (ascorbic acid, pyridoxine hydrochloride, pyridoxal hydrochloride, pyridoxamine dihydrochloride, p-aminobenzoic acid, folic acid) by liquid chromatography, with a parallel dual-electrode electrochemical detector, is described. One electrode was controlled at +0.80 V ( vs. SCE), the other at +1.20 V ( vs. SCE). The possibility of interference by eight other water-soluble vitamins (riboflavin, nicotinamide, cyanocobalamin, menadione, dextro calcium pantothenate, thiamine, nicotinic acid, dextro biotin) was studied. These vitamins did not interfere when a parallel dual-electrode detector system was used. The estimation of five of the vitamins was studied in detail. The linear ranges found were 10 ng—1.2 μg for pyridoxine hydrochloride, 2 ng—2μg for pyridoxal hydrochloride, 10 ng-3 μg for pyridoxamine dihydrochloride, 5–200 ng for folic acid and 0.6–200 ng for p-aminobenzoic acid, the limits of detection being 3, 0.6, 1, 2 and 0.06 ng respectively. Application of the technique to the estimation of vitamin B 6 in tablets is illustrated. The results indicate that the vitamin B 6 in these tablets existed in the pyridoxine hydrochloride form and the B 6 content agreed well with liquid chromatograph by spectrophotometric analysis.
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