Determination of Vitamin-B6 by Vanadium (III) Schiff base complex modified GCE

Abstract

Vitamin B6 (pyridoxine) is an important vitamin for both mental and physical health processes. Vitamin B6 (V-B6) is much essential for the formation of red blood cells. It takes up wide variety of functions in human body in maintaining good health. The conversion of tryptophan amino acid to niacin vitamin needs the V-B6. V-B6 is an important vitamin for the nervous and immune system functions. V-B6 is an essential vitamin for the better health condition of human body. The deficiency of V-B6 can causes anaemia that is similar to iron deficiency anaemia. Due to these health factors, it is necessary to develop a simple, selective and sensitive method for the analysis of V-B6. There are several analytical methods available for the quantification of V-B6, such as flow HPLC, electrophoresis, spectrophotometric method, fluorimetric method and electrocatalytic detection. The electrocatalytic method gives good accuracy, faster response, simple procedure and cost effective, so the electrocatalytic sensing was chosen for the determination of V-B6. In the present work, we have used voltammetric method for the determination of V-B6 using modified GCE. For the GCE active surface modification we have utilized vanadium(III) Schiff base complex. Vanadium has received considerable interest biologically and chemically, due to its various stable oxidation states +3, +4, +5. Due to different oxidation states, vanadium complexes show good electrochemical redox behaviour. The vanadium Schiff base complex was electrochemically polymerized using the GCE as working electrode. The polymer vanadium complex was deposited on the GCE surface. The modified GCE exhibits the oxidation peak for V-B6 at 1.212 V and the anodic peak current 9.21 μA. The bare GCE shows the anodic peak at 1.236 V with the peak current is 8.49 μA. These results clearly indicate that the vanadium Schiff base complex modified GCE has better electrocatalytic sensing ability for the determination of vitamin B6.© 2017 Elsevier Ltd. All rights reserved.Selection and/or Peer-review under responsibility of International Conference On Recent Advances In Material Chemistry.