Abstract
Vitamin B12 is a type of vitamin also known by the name cobalamin. B12 is involved in many metabolism activities, including DNA synthesis, nervous system, red blood formation and immune system. Therefore, we chose the Differential Pulse Polarography (DPP) method is that has a high sensitivity for the determination of vitamin B12. This determination was possible with cobalt present in vitamin B12 structure. Since Co(III) is formed from the oxidation of the vitamin, its polarographic behavior had to be determined in various electrolytes such as acetate, borate, phosphate and ammonia. The polarograms of Co(III) were taken in these electrolytes in which 1.0 M NH3/ (pH = 9.8) and 1.0 M AcOH/AcO- (pH = 4.0) were found as the most suitable electrolytes. This method was successfully applied vitamin of B12 determination in a 1 mL ampoule with high precision. The LOD was found as 3.7 × 10-7 for instead of (S/N = 3). Besides Co(III), interference effects of Zn(II), Ni(II), Cr(III), Fe(III), Cu(II), Cd(II) and Se(IV) were also studied. It was found that only Zn(II) peak had an overlap Co(III) peak in ammonium buffer. This problem could be solved by working in 1.0 M AcOH/AcO- (pH = 4.0) buffer. B12, which is 1000 μg in 1 mL vitamin ampoule, was found for 4 measurements as 999 ± 15 μg as a result of 95% confidence interval.
Highlights
Vitamin B12 is a type of vitamin known as cobalamin, and it is a water-soluble vitamin like other B vitamins
Preparation of B12 vitamin solution: Since Cobalt is present in the organic structure of vitamin B12, it had to be decomposed with concentrated acid mixture as given below, so that the organic structure was decomposed and Co(III) ions could be dissolved in the solution
Since cobalt ion is present in vitamin B12 as Co(III) after destruction with acids, the best medium for its determination had to be studied
Summary
Vitamin B12 is a type of vitamin known as cobalamin, and it is a water-soluble vitamin like other B vitamins. Electrochemical methods are preferred because of their high sensitivity and low interference effect in the determination of cobalt. It is a method that does not require enrichment and extraction in DPP for the determination of trace amounts of elements These kinds of determinations are rarely possible in many analytical methods [1] [2]. B12 in vitamin B12 tablets were determined by detection by a high performance liquid chromatography [12]. Trace Cobalt in vitamin B12 is determined by polarography using a complex of Co(II)-thymolphthaleinnitrite. With this adsorptive wave it was possible to determine cobalt in vitamin B12 [18]. High sensitivity differential pulse polarography was used In this analysis, the interference effects of other cations were examined. The appropriate medium for vitamin B12 determination was investigated and the vitamin B12 in the ampoule was analyzed in this medium
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