Abstract
Objective: To determine total phenolic and flavonoids contents and also quantify vindoline and rutin in different morphotypes of Catharanthus roseus using High-performance liquid chromatography (HPLC) method.
 Methods: Total flavonoids content (TFC) was determined by Aluminium chloride colorimetric and total phenolic content (TPC) was estimated by Folin-Ciocalteu reagent assay. The chromatographic separation was done by using a C18 column at room temperature and eluted with a mobile phase consisting of a mixture of phosphate buffer (pH=5.8) and acetonitrile at a flow rate of 1.0 ml/minute and detection was carried out at 254 nm.
 Results: TPC and TFC content was found highest in Cr00DP and lowest in Cr00WFSRE. Results also showed that the purple morphotypes Cr00DP gives more vindoline (0.3 mg/g) and rutin (18.57 mg/g) concentration compared to the pink morphotype Cr00PFRE contained 18.3 mg/g rutin and 0.2 mg/g vindoline. White morphotypes contained 0.383 mg/g rutin and 0.004 mg/g vindoline which was significantly less as compared to purple and pink morphotypes.
 Conclusion: The plant has significant number of alkaloids and flavonoids. The obtained outcomes from different morphotypes are thus significant for the purpose of vindoline and rutin isolation from Catharanthus roseus plant. These isolated bioactive phytoconstituents are a good candidate for further pharmacological and clinical study.
Highlights
Plants, since time perpetual, have been exploited for their therapeutic potential and play a critical job in the prosperity of man
Plants have a wide assortment of phytocompounds like alkaloids, phenols, flavonoids, glycosides, and tannins, etc. which are responsible for the various biological properties and amelioration of human beings
The results accessed from preliminary phytochemical analysis of the crude leaf extracts of different accession of C. roseus are presented in
Summary
Since time perpetual, have been exploited for their therapeutic potential and play a critical job in the prosperity of man. Researchers are showing incredible interest in plant-derived substances because of their versatile applications [1]. Plants are one of the richest bio-resources of current medicines, nutraceutical, food supplements, folk medicines, pharmaceutical intermediates and chemical entities for synthetic drugs [2]. Plants have a wide assortment of phytocompounds like alkaloids, phenols, flavonoids, glycosides, and tannins, etc. Which are responsible for the various biological properties and amelioration of human beings. Plants with antimicrobial activities have become more interesting because of the emergence of multiple drug-resistant strains of microorganisms. Due to indiscriminate and over-prescription of some antibiotics have generated a renewed interest in herbal medicines [3]
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