Abstract
A gas-liquid chromatography technique has been developed for the quantitative determination of convicine and vicine in fababeans. The trimethylsilyl derivatives of these glycosides were readily separated on an OV-17 liquid phase at 230°C. Mass spectra (electron impact ionization) of the peaks obtained in the GLC analysis of the fababean extract which corresponded in retention time to authentic convicine and vicine were identical to those of the authentic glycosides. Treatment of the fababean extract with β-glucosidase resulted in virtually complete disappearance of the components that corresponded in retention time to the authentic glycosides, indicating the absence of co-eluting impurities. Significant varietal differences in convicine and vicine and total glycoside content were found, indicating that plant breeding and selection for low glycoside content may be effective.
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Schedule a callMore From: Canadian Institute of Food Science and Technology Journal/Journal de L'Institut Canadien de Science et Technologie Alimentaire
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