Abstract
A individual approach to the treatment of cancer patients, which takes the genetic features of the patient’s metabolism into account, allows one to choose an appropriate drug and its dose, that increases the efficiency of therapy and prevents the development of side toxic effects. For this purpose, we developed a biological microarray-based method for studying the primary structure of patient DNA and identifying polymorphisms in the UGT1A1, DPYD, GSTP1, and ABCB1 genes associated with alterations in xenobiotic metabolism. Genotyping of samples from 89 cancer patients and 15 healthy donors was carried out using the developed microarray. The results of determination of the primary structure of DNA samples coincided completely with the control sequencing. To increase the specificity of determination of polymorphic variants of the UGT1A1 gene, we used hybridization probes containing LNA nucleotides. The frequencies of polymorphic allelic variants of the UGT1A1*28, DPYD*2A, GSTP1 (I105V), and ABCB1 (C3435T) genes in the studied sample were 0.39, 0, 0.33, and 0.57, respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
